An early warning system that samples and tests Saskatoon’s wastewater for the SARS-CoV-2 virus, which causes COVID-19, has been developed by University of Saskatchewan researchers, the City of Saskatoon, and the Saskatchewan Health Authority.

Infected people shed virus traces through their feces, often even before COVID-19 symptoms are apparent. Using a technique developed at USask, the team has found that changes to the total amount of virus circulating in the City’s wastewater happen about one week ahead of changes indicated by case counts at COVID testing centres.

“We think we can give health officials at least a week’s notice on changes in the trend line,” said John Giesy, ecotoxicologist at the University of Saskatchewan (USask). “Based on the latest data, which shows the trend line is going up, I am predicting we will see a rise in cases for the next couple of weeks. We can also predict when outbreaks are declining, which will help planning for pandemic recovery.”

At present, the team can only predict the trend in COVID cases—up or down, but not specifics on how big a rise or fall in cases might be.

“It’s really about comparing trends in test cases with wastewater virus concentrations,” said Markus Brinkmann, ecotoxicologist at USask. “If the virus concentration in the wastewater swings up before we see an increase in COVID test cases, we would expect the curve of the test cases to increase in the upcoming week. Over the past two weeks, we have seen an exponential increase in virus copies in the wastewater.”

The team’s wastewater surveillance approach is based on a method for measuring SARS-CoV-2 virus environmental degradation developed by Giesy and Yuwei Xie. Working with USask engineering researcher Kerry McPhedran, the team has adapted the method to capture COVID-19 information. Engineering graduate student Shahab Minaei and his supervisor Jafar Soltan are also part of the team.

Monitoring of the prevalence of the SARS-CoV-2 virus in Saskatoon’s wastewater began in July 2020. At the city’s wastewater treatment plant, samples are automatically collected over a 24-hour period and pumped into a refrigerator to preserve the quality of the sample. It is treated with UV lights before the wastewater is released into the environment, which neutralizes any potential infective virus according to USask.

The sample is brought to labs on campus where Xie undertakes a two- to three-day analysis of the purified wastewater. Xie counts the number of copies of an RNA sequence specific to SARS-CoV-2 to establish a total concentration of virus in the sample.

“We can anticipate the rate of change in cases so that public health measures can be implemented in response,” said Brinkmann. “Epidemiologists get more accurate information about prevalence and health officials get a jump-start on allocating resources effectively.”

The research team produces data once per week, and then shares it with the City of Saskatoon which conveys it to the Saskatchewan Health Authority.

“The SHA is pleased to be a partner since the inception of this research project, and this is another tool in our surveillance system kit to assist the health system in making decisions on where to focus efforts,” said Dr. Simon Kapaj, Saskatchewan Health Authority’s medical health officer of environmental public health. “The early findings are shaping the public health response in Saskatoon and we believe this tool could assist other major cities in Saskatchewan.”

The research is currently funded by the USask-led Global Water Futures program, which is supported by the Canada First Research Excellence Fund. The project was originally intended to measure environmental contaminants but pivoted to focus on COVID-19 research. The work is also supported by in-kind contributions of equipment and personnel from the City of Saskatoon.

“The City of Saskatoon is pleased to have formed this partnership with the University of Saskatchewan team and the Saskatchewan Health Authority to help understand the prevalence of COVID-19 in the community,” said Mike Sadowski, wastewater treatment plant manager at the City of Saskatoon.

Brinkmann said the research team hopes to expand their partnership with City of Saskatoon and the SHA Saskatchewan Health Authority provide more frequent estimates (three times per week) and expand to more cities in Saskatchewan.

“Right now, we’re limited in what data we can produce,” said Brinkmann. “If we had dedicated funding for this, we could increase the frequency of gathering and analyzing samples to three times a week, giving us more detailed information with which to perform a statistical analysis for potentially predicting new case numbers.”

The USask approach has been validated by reliability testing organized by the Canadian Water Network (CWN). Using a set of samples with known concentrations of the virus, laboratories across Canada compared different methods of analysis and found the USask-developed method was accurate in detecting the virus at different concentrations. Giesy is a member of the CWN advisory panel which also includes virologists, epidemiologists, and engineers.


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